期刊
CHINESE CHEMICAL LETTERS
卷 31, 期 5, 页码 1104-1108出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2019.10.021
关键词
DNA metabolic labeling; Bioorthogonal chemistry; Photocage group; Modified nucleoside; Cell imaging
资金
- National Natural Science Foundation of China [21432008, 91753201, 21721005]
Bioorthogonal cleavage and ligation reactions together form one more integrated system about the repertoire of bioorthogonal chemistry, capacitating an array of thrilling new biological applications. The bond-cleavage type and position of biomolecular remain a great challenge, which determines the metabolic pathway of the targets in living systems. Herein we designed two linkages of methylene and carbonyl group attached the N-3 position of the 5-ethynyl-2'-deoxyuridine (EdU) base or the oxygen atom at deoxyribose 3' position to a photocaging group, which would be cleaved by irradiation with 365 nm ultraviolet light. EdU derivatives linked by methylene at the N-3 position had better photodecage efficiency and stability in the absence of light. This paper provides a strategy for studying the nucleoside metabolic pathways in cells, which can easily and conveniently evaluate the effect of the position and type of the linkages. The developed strategy affords a reference for controlling spatial and temporal metabolism of small-molecule drugs, allowing direct manipulation of intact cells under physiological conditions. (C) 2019 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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