Synthesis, characterization and spectroscopic studies of surfactant loaded antiulcer drug into Chitosan nanoparticles for interaction with bovine serum albumin

In the present study, Lansoprazole loaded Chitosan-Tween80 Nanoparticles (NLPZ) have been synthesized by ionotropic gelation method and characterized by FTIR, PXRD, DLS, SEM and UV-Vis techniques. The intermolecular interactions of LPZ and NLPZ with BSA were investigated using fluorescence, UV-vis, circular dichroism, and molecular docking techniques. The spectroscopic techniques suggested effective binding of LPZ and NLPZ to BSA under physiological conditions. The S-V plot of BSA-LPZ system showed positive deviation high-lighting the presence of both static and dynamic quenching. Hence, ground state complex model and sphere of action quenching model were used for the study of BSA-LPZ system. The K-SV and Ka of BSA-LPZ system were higher as compared to BSA-NLPZ system. Thermodynamic parameters indicated that the binding reaction in both systems was exothermic and spontaneous, enthalpically driven and also hydrogen binding and Van der Waals forces played a major role in the interaction of BSA-LPZ and BSA-NLPZ systems.