期刊
CELL PROLIFERATION
卷 53, 期 1, 页码 -出版社
WILEY
DOI: 10.1111/cpr.12697
关键词
bone remodelling; mineralized tissue; osteoblast; osteoclast
类别
资金
- National Natural Science Foundation of China [81771030]
- Construction Engineering Special Fund of TaishanScholars [tsqn20161068, ts201511106]
Objectives miR-21 can promote osteoblast differentiation of periodontal ligament stem cells. However, the effect of miR-21 on bone remodelling in the midpalatal suture is unclear. This study aimed to elucidate the effects of miR-21 on the midpalatal suture bone remodelling by expanding the palatal sutures. Materials and methods miR-21 deficient (miR-21(-/-)) and wild-type (WT) mice were used to establish animal models by expanding the palatal sutures. Micro-CT, haematoxylin-eosin (HE) staining, tartrate-resistant acid phosphatase (TRAP) staining, fluorescence labelling and immunohistochemistry were used to investigate the function of miR-21 in midpalatal suture bone remodelling. Besides, bone mesenchymal stem cells (BMSCs) derived from both miR-21(-/-) and WT mice were cultured. The MTT, CCK8, EdU analysis, transwell and wound healing test were used to assess the effects of miR-21 on the characteristics of cells. Results The expression of ALP was suppressed in miR-21(-/-) mice after expansion except 28 days. The expression of Ocn in WT mice was much higher than that of miR-21(-/-) mice. Besides, with mechanical force, miR-21 deficiency downregulated the expression of Opg, upregulated the expression of Rankl, and induced more osteoclasts as TRAP staining showed. After injecting agomir-21 to miR-21(-/-) mice, the expression of Alp, Ocn and Opg/Rankl were rescued. In vitro, the experiments suggested that miR-21 deficiency reduced proliferation and migration ability of BMSCs. Conclusions The results showed that miR-21 deficiency reduced the rate of bone formation and prolonged the process of bone formation. miR-21 regulated the bone resorption and osteoclastogenesis by affecting the cell abilities of proliferation and migration.
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