期刊
CANCER RESEARCH
卷 80, 期 2, 页码 319-333出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-19-1023
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资金
- National Science Foundation of China [31330023, 81722021, 31671483, 81771627, 31521003, 31821002, 91753207, 31930062, 81802525, 81672544, 81872099, 31871432]
- Shanghai Rising-Star Program [18QA1400300]
- National Key R&D Program of China [2018YFA0800300, 2019YFA0801900, 2018YFA0801300, 2018YFC1004700]
- Science and Technology Municipal Commission of Shanghai, China [16JC1405301, 16JC1405302, 18511108000, 2018ZHYL0201]
- Shanghai Sailing Program [17YF1402700]
- Shanghai Natural Science Foundation of China [16ZR1406400]
- Shanghai Rising Stars of Medical Talent Youth Medical Talents-Specialist Program
tumorigenic role and underlying mechanisms of lipid accumulation, commonly observed in many cancers, remain insufficiently understood. In this study, we identified an AMP-activated protein kinase (AMPK)-GATA-binding protein 3 (GATA3)-enoyl-CoA hydratase short-chain 1 (ECHS1) pathway that induces lipid accumulation and promotes cell proliferation in clear cell renal cell carcinoma (ccRCC). Decreased expression of ECHS1, which is responsible for inactivation of fatty acid (FA) oxidation and activation of de novo FA synthesis, positively associated with ccRCC progression and predicted poor patient survival. Mechanistically, ECHS1 downregulation induced FA and branched-chain amino acid (BCAA) accumulation, which inhibited AMPK-promoted expression of GATA3, a transcriptional activator of ECHS1. BCAA accumulation induced activation of mTORC1 and de novo FA synthesis, and promoted cell proliferation. Furthermore, GATA3 expression phenocopied ECHS1 in predicting ccRCC progression and patient survival. The AMPK-GATA3-ECHS1 pathway may offer new therapeutic approaches and prognostic assessment for ccRCC in the clinic. Significance: These findings uncover molecular mechanisms underlying lipid accumulation in ccRCC, suggesting the AMPK-GATA3-ECHS1 pathway as a potential therapeutic target and prognostic biomarker.
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