期刊
BIOLOGICAL CHEMISTRY
卷 397, 期 1, 页码 37-44出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/hsz-2015-0161
关键词
biosensors; excluded volume; macromolecular crowding; protein stability; quinary interactions
资金
- Ministry of Innovation, Science and Research of the State of North Rhine-Westphalia (Ruckkehrerprogramm)
- Cluster of Excellence RESOLV - German Research Foundation (DFG) [EXC 1069]
- International Graduate School of Neuroscience (Ruhr-University Bochum, Germany)
The influence of the cellular milieu, a complex and crowded solvent, is often neglected when biomolecular structure and function are studied in vitro. To mimic the cellular environment, crowding effects are commonly induced in vitro using artificial crowding agents like Ficoll or dextran. However, it is unclear if such effects are also observed in cellulo. Diverging results on protein stability in living cells point out the need for new quantitative methods to investigate the contributions of excluded volume and nonspecific interactions to the cellular crowding effect. We show how new crowding sensitive probes may be utilized to directly investigate crowding effects in living cells. Moreover, we discuss processes where crowding effects could play a crucial role in molecular cell biology.
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