4.3 Article

Cleavage of Target DNA Promotes Sequence Conversion with a Tailed Duplex

期刊

BIOLOGICAL & PHARMACEUTICAL BULLETIN
卷 39, 期 8, 页码 1392-1395

出版社

PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.b16-00325

关键词

sequence conversion; tailed duplex; genome editing

资金

  1. Japan Society for the Promotion of Science KAKENHI Grant [25282144]
  2. Grants-in-Aid for Scientific Research [25282144] Funding Source: KAKEN

向作者/读者索取更多资源

Base sequence conversion in target DNA is achieved when a 5'-tailed duplex (TD) is introduced into cells. In this study, the effects of target DNA cleavage on sequence conversion with a TD were examined. Plasmid DNAs with and without cleavage near the target position were each introduced into HeLa cells, together with the TD. The cleavage promoted the sequence alteration efficiency by ca. 7-fold. These results suggested that the sequence conversion efficiency with the TD fragment is increased when an artificial nuclease introduces cleavage near the target site.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据