期刊
BMC MEDICINE
卷 17, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12916-019-1439-x
关键词
NG2 glia; Neuroinflammation; Microglia; TGF-beta; CX3CR1; Parkinson's disease
资金
- Natural Science Foundation of China [31430036, 91742116, U1801681]
- National Key Basic Research Program of China [2015CB553500]
- Key Research Program of Frontier Sciences [QYZDJ-SSW-SMC002]
- Strategic Priority Research Program of Chinese Academy of Science [XDB32020100]
- Shanghai Municipal Science and Technology Major Project [2018SHZDZX05]
- Guangdong Provincial Key RD Program [2018B030337001]
- Shanghai Municipal Science and Technology Commission [17ZR1435300, Y75EN21241]
- Shandong Province Higher Educational Science and Technology Program [J18KA124]
- Innovative Research Team of High-Level Local Universities in Shanghai
Background Brain innate immunity is vital for maintaining normal brain functions. Immune homeostatic imbalances play pivotal roles in the pathogenesis of neurological diseases including Parkinson's disease (PD). However, the molecular and cellular mechanisms underlying the regulation of brain innate immunity and their significance in PD pathogenesis are still largely unknown. Methods Cre-inducible diphtheria toxin receptor (iDTR) and diphtheria toxin-mediated cell ablation was performed to investigate the impact of neuron-glial antigen 2 (NG2) glia on the brain innate immunity. RNA sequencing analysis was carried out to identify differentially expressed genes in mouse brain with ablated NG2 glia and lipopolysaccharide (LPS) challenge. Neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice were used to evaluate neuroinflammatory response in the presence or absence of NG2 glia. The survival of dopaminergic neurons or glial cell activation was evaluated by immunohistochemistry. Co-cultures of NG2 glia and microglia were used to examine the influence of NG2 glia to microglial activation. Results We show that NG2 glia are required for the maintenance of immune homeostasis in the brain via transforming growth factor-beta 2 (TGF-beta 2)-TGF-beta type II receptor (TGFBR2)-CX3C chemokine receptor 1 (CX3CR1) signaling, which suppresses the activation of microglia. We demonstrate that mice with ablated NG2 glia display a profound downregulation of the expression of microglia-specific signature genes and remarkable inflammatory response in the brain following exposure to endotoxin lipopolysaccharides. Gain- or loss-of-function studies show that NG2 glia-derived TGF-beta 2 and its receptor TGFBR2 in microglia are key regulators of the CX3CR1-modulated immune response. Furthermore, deficiency of NG2 glia contributes to neuroinflammation and nigral dopaminergic neuron loss in MPTP-induced mouse PD model. Conclusions These findings suggest that NG2 glia play a critical role in modulation of neuroinflammation and provide a compelling rationale for the development of new therapeutics for neurological disorders.
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