期刊
BIOTECHNOLOGY LETTERS
卷 42, 期 2, 页码 269-276出版社
SPRINGER
DOI: 10.1007/s10529-019-02771-6
关键词
Immobilization; Candida antarctica lipase B; Zeolitic imidazole framework; Macroporous resin; Characterization
资金
- National Natural Science Foundation of China [31660247, 31600639] Funding Source: Medline
- Key Research and Development Program of Zhejiang Province [2019C01082] Funding Source: Medline
A novel enzyme immobilization method employing metal-organic framework (MOF) encapsulation and macroporous resin adsorption was developed in this study. Candida antarctica lipase B (CALB) was firstly encapsulated onto metal-organic frame structures (Zeolitic imidazole framework-8, ZIF-8) and further bonded to macroporous resin by physical adsorption. Under optimized immobilization conditions, the activity of the prepared immobilized lipase (CALB-ZIF-8@D101) determined via the methyl esterification of oleic acid was 38.4 U/mg. Compared with free lipase, the immobilized lipase exhibited improved thermal and operational stability and organic solvent tolerance. These results demonstrate that the immobilization method of ZIF-8 encapsulation and macroporous resin adsorption enhanced enzyme properties at a superior level.
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