期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 67, 期 2, 页码 240-248出版社
WILEY
DOI: 10.1002/bab.1840
关键词
gamma-aminobutyric acid; glutamate decarboxylase; heterologous expression; Saccharomyces cerevisiae; whole-cell bioconversion
资金
- National Natural Science Foundation of China [31741102, 31871763] Funding Source: Medline
- Natural Science Foundation of Zhejiang Province [LY14C200007] Funding Source: Medline
- Science and Technology Department of Zhejiang Province [2017C02033, 2018C02053] Funding Source: Medline
- Hangzhou Science and Technology Bureau [171593] Funding Source: Medline
Gamma-aminobutyric acid (GABA) is an industrially valuable natural product. This study was aimed to establish an efficient food-grade production process of GABA by engineeringSaccharomyces cerevisiaethat is generally recognized as safe (GRAS). GABA can be produced by catalytic decarboxylation ofl-glutamate (l-Glu) by glutamate decarboxylase (GAD, EC4.1.1.15). Two GADs, SsGAD fromStreptomyces sp. MJ654-NF4 and ScGAD fromStreptomyces chromofuscusATCC 49982, were heterologously expressed inS. cerevisiaeBJ5464. The engineered yeast strains were used as whole-cell biocatalysts for GABA production.S. cerevisiaeBJ5464/SsGAD exhibited significantly higher efficient catalytic activity than that ofS. cerevisiaeBJ5464/ScGAD. The optimal bioconversion system consisted of a cell density of OD(600)30, 0.1 Ml-Glu, and 0.28 mM pyridoxal phosphate in 0.2 M Na2HPO4-citric acid buffer with pH 5.4, and the reactions were performed at 50 degrees C for 12 H.S. cerevisiaeBJ5464/SsGAD cells can be reused, and the accumulated GABA titer reached 62.6 g/L after 10 batches with an overall molar conversion rate of 60.8 mol%. This work thus provides an effective production process of GABA using engineered yeast for food and pharmaceutical applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据