期刊
BIOTECHNIQUES
卷 68, 期 1, 页码 7-13出版社
FUTURE SCI LTD
DOI: 10.2144/btn-2019-0066
关键词
bacteria; cell viability; flow cytometry; membrane-exclusion dye; microscopy; vital dye
Rapidly assaying cell viability for diverse bacteria species is not always straightforward. In eukaryotes, cell viability is often determined using colorimetric dyes; however, such dyes have not been identified for bacteria. We screened different dyes and found that erythrosin B (EB), a visibly red dye with fluorescent properties, functions as a vital dye for many Gram-positive and -negative bacteria. EB worked at a similar concentration for all bacteria studied and incubations were as short as 5 min. Given EB's spectral properties, diverse experimental approaches are possible to rapidly visualize and/or quantitate dead bacterial cells in a population. As the first broadly applicable colorimetric viability dye for bacteria, EB provides a cost-effective alternative for researchers in academia and industry. METHOD SUMMARY Incubation of erythrosin B with bacteria specifically stains membrane-compromised dead cells. Live and dead cells can then be quantified via bright-field microscopy or any number of instruments that quantitate fluorescence.
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