期刊
BIOTECHNIQUES
卷 67, 期 4, 页码 188-191出版社
FUTURE SCI LTD
DOI: 10.2144/btn-2019-0071
关键词
cell-free DNA; DNA purification; liquid biopsy; next-generation sequencing; SPRI beads
资金
- Ripple Biosolutions LLC
Genomic analysis of circulating, cell-free DNA (cfDNA) is being used extensively for molecular diagnostics. Many approaches rely on the construction of cfDNA genomic libraries, targeted retrieval of specific genomic regions and analysis by next-generation DNA sequencing. Several steps during sample preparation require isolation of DNA fragments within a particular size range. In this Benchmark article, two related methods for size-selective DNA fragment enrichment are described.
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