期刊
BIORESOURCE TECHNOLOGY
卷 298, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.122511
关键词
Lignin-first biorefinery; Thermostable laccase; Metagenome; Heterologous expression; Direct evolution
资金
- National Key Research and Development Program of China [2017YFC0212205]
- National Natural Science Foundation of China [21876030]
- International Cooperation Project of Shanghai Municipal Science and Technology Commission [18230710700]
As the most abundant aromatic polymers on the Earth, lignin has great potential to produce biofuels and aromatic chemicals due to their high carbon content and low oxygen content. Lignin-first biorefinery methods have attracted increasing attention recently for their high-value of aromatic chemicals, and high biofuels productivity from lignocellulosic wastes. Thermostable laccase has proven to be an excellent alternative catalyst in degrading lignin for its versatile catalytic abilities under industrial conditions and pollution-free by-products. Thermostable laccases can be found in native extreme environments or modified by biologically based technologies such as gene recombination expression and enzyme direct evolution. This review demonstrated thermostable laccases and their application in lignin degradation. Future research should focus more on the investigation of the reaction of thermostable laccases with lignin substrates.
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