Enhancement of the ANAMMOX bacteria activity and granule stability through pulsed electric field at a lower temperature (16 ± 1 °C)

The effects of different frequencies of pulsed electric field (PEF) on the ANAMMOX process were investigated. The results showed that the intermediate frequency could dramatically enhance both the ANAMMOX bacterial activity and granule sludge stability at 16 +/- 1 degrees C The nitrogen removal efficiency of R1 (intermediate frequency) was significantly enhanced by 62.24% and 79.51% compared to R2 (lower frequency) and R3 (higher frequency), with a nitrogen loading rate of 6.84 kg Nm(-3) d(-1). In addition, the intermediate frequency could stimulate cells to secrete more extracellular polymeric substances (EPS) to sustain the granule sludge stability. The granule sludge disintegrated on days 55 and 35 in R2 and R3. The protein (PN)/polysaccharide (PS) ratios of R1 were 28.46% and 54.20% higher than R2 and R3, which was beneficial to granule sludge stability. This study showed that PEF could solve the problem of decreased ANAMMOX bacterial activity and granule stability at lower temperatures.