期刊
BIORESOURCE TECHNOLOGY
卷 289, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.121755
关键词
Xylooligosaccharides; Gluconic acid; Sugarcane bagasse; Enzymatic hydrolysis; Gluconobacter oxydans
资金
- National Key R&D Program of China [2017YFD0601001, 163030127]
A novel approach was proposed for the production of xylooligosaccharides by direct pre-hydrolysis using gluconic acid as catalyst. Maximum xylooligosaccharides (degree of polymerization 2-6) yield of 53.2% could be obtained in 60 min through 5% gluconic acid hydrolysis of sugarcane bagasse at 150 degrees C. Furthermore, the yield of glucose from solids following gluconic acid hydrolysis treatment was 86.2% after fed-batch enzymatic hydrolysis with 10% solids loading. Results indicated that gluconic acid pretreatment combined with enzymatic hydrolysis could be successfully applied to sugarcane bagasse substrate. Subsequently, glucose could be efficiently bio-oxidized to gluconic acid by Gluconobacter oxydans ATCC 621H with 93.1% yield, and sugarcane bagasse derived gluconic acid has been proved to be an effective catalyst for xylooligosaccharides production. In this study, xylooligosaccharides production from sugarcane bagasse by gluconic acid hydrolysis demonstrated a great potential with respect to the production of these probiotics around the world.
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