期刊
BIORESOURCE TECHNOLOGY
卷 289, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.121612
关键词
Epimedin C; Icariin; alpha-L-Rhamnosidase; Bioconversion; Aspergillus nidulans
资金
- National Key Research and Development Program of China [2017YFC1600403, 2018YFC1604102]
- National Science Fund for Excellent Young Scholars [21822806]
- National Natural Science Foundation of China [31670095, 31770097]
- China Postdoctoral Science Foundation [2019M651701]
- Fundamental Research Funds for the Central Universities [JUSRP51701A]
- National First-class Discipline Program of Light Industry Technology and Engineering [LITE2018-08]
- Distinguished Professor Project of Jiangsu Province
Herba Epimedii is a traditional Chinese herbal medicine that contains a mixture of bioactive flavonoid glycosides. Among them, icariin has the most outstanding bioactive functions, while epimedin C exhibits substantial toxicity. A recombinant alpha-L-rhamnosidase ((syn)AnRhaE) from Aspergillus nidulans was expressed in Escherichia coli to promote the efficient bioconversion of epimedin C to icariin. A hydrolase activity of 574.5 U L-1 was acquired via optimized fed-batch fermentation in a 5-L bioreactor. The enzyme proved to be stable in an acidulous pH range below 55 degrees C with an optimal pH of 4.5 and optimal temperature of 55 degrees C. Epimedin C (1 g L-1) was 100% converted to icariin within 90 min using recombinant cells. The resting cells proved to be selective for epimedin C and 2 ''-O-rhamnosylicariside II in crude extracts of the epimedium plant. This work provides an original and efficient biocatalyst system that can be applied in industrialized production of icariin.
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