期刊
BIORESOURCE TECHNOLOGY
卷 289, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.121673
关键词
D-Allulose; D-Tagatose-3-epimerase; Polyhydroxyalkanoates; Immobilization; Low-calorie sweetener
资金
- National Natural Science Foundation of China [21306110, 21602171]
- Scientific Research Projects of Shaanxi Academy of Science [2017 K-13, 2019 K-09, 2018NK-05]
- Natural Science Foundation of Shaanxi Province [2017JM2034]
D-Allulose is a promising low-calorie sweetener especially for diabetes and obesity patients. The functionalized polyhydroxyalkanoate (PHA) nano-beads decorated with D-tagatose 3-epimerase (DTE) was produced in recombinant endotoxin-free ClearColi, whereby the expression, purification, and immobilization of the active DTE were efficiently combined into one step. The immobilized DTE exhibited remarkable enzyme activity of 649.3 U/g beads and extremely high stability at a harsh working condition (pH 7.0-8.0, 65 degrees C). When DTE-PHA beads were subjected to enzymatic synthesis of D-allulose, a maximum conversion rate of 33% can be achieved at pH 7.0 and 65 degrees C for 3 h, and DTE-PHA beads retained about 80% of its initial activity after 8 continuous cycles. Moreover, the D-allulose/D-fructose binary mixture can be simply separated by a single cation exchange resin-equipped chromatography. Taken together, DTE-PHA beads are promising and robust nano-biocatalysts that will remarkably simplify the production procedures of D-allulose, contributing to its cost-effective production.
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