期刊
BIOPHYSICAL CHEMISTRY
卷 253, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bpc.2019.106217
关键词
GUVs; Electroformation; Electric potential; Frequency; Temperature; Response surface methodology
资金
- National Natural Science Foundation of China [21773050]
- Natural Scienee Foundation of Heilongjiang Province for Distinguished Young Scholars [JC2018003]
The production of giant unilamellar vesicles (GUVs) with specific size and structure has been a challenge on the design of quantitative biological assays in cell-mimetic micro-compartments. In this study, the effect of electroformation parameters (electric potential, frequency, and temperature) on the size of GUVs was investigated. Using response surface methodology based on Box-Behnken design, GUVs from neutral, positive and negative charges were formulated. The average diameter of GUVs was determined for each formulation. The acquired data of these GUVs were successfully fitted with quadratic regression models. These models were applied to visualize the parameters for ideal GUVs with wanted diameters by the obtained phase diagrams. These results show that response surface methodology can be used to estimate the electroformation parameters for specifically sized GUVs.
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