期刊
BIOMATERIALS
卷 219, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2019.119357
关键词
Nanoparticle; Protein corona; Zwitterion
资金
- Agence Nationale de le Recherche (LocalEndoProbes grant) [ANR-17-CE16-0012-02]
- European Union [686841]
- French National Research Agency (ANR) Paris-Science-Lettres Program [ANR-10-IDEX-0001-02 PSL]
- French National Research Agency (ANR) Labex CelTisPhyBio [ANR-10-LBX-0038]
- Ecole Doctorale Frontieres du Vivant (FdV) - Programme Bettencourt
- Agence Nationale de la Recherche (ANR) [ANR-17-CE16-0012] Funding Source: Agence Nationale de la Recherche (ANR)
In the last few years, zwitterionic polymers have been developed as antifouling surface coatings. However, their ability to completely suppress protein adsorption at the surface of nanoparticles in complex biological media remains undemonstrated. Here we investigate the formation of hard (irreversible) and soft (reversible) protein corona around model nanoparticles (NPs) coated with sulfobetaine (SB), phosphorylcholine (PC) and carboxybetaine (CB) polymer ligands in model albumin solutions and in whole serum. We show for the first time a complete absence of protein corona around SB-coated NPs, while PC- and CB-coated NPs undergo reversible adsorption or partial aggregation. These dramatic differences cannot be described by naive hard/soft acid/base electrostatic interactions. Single NP tracking in the cytoplasm of live cells corroborate these in vitro observations. Finally, while modification of SB polymers with additional charged groups lead to consequent protein adsorption, addition of small neutral targeting moieties preserves antifouling and enable efficient intracellular targeting.
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