期刊
BIOCHEMICAL JOURNAL
卷 476, 期 -, 页码 3069-3080出版社
PORTLAND PRESS LTD
DOI: 10.1042/BCJ20190483
关键词
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资金
- National Natural Science Foundation of China [31572264, 31071922]
- Innovative Research Team (in Science and Technology) at the University of Henan Province [19IRTSTHN008]
- National Engineering Laboratory for Wheat & Corn Further Processing, Henan University of Technology [NL2016010]
CGA-N9, a peptide derived from human chromogranin A (CGA), was found to have antimicrobial activity in our previous investigation, but its mechanism of action remains unclear. Herein, the mechanism of action of CGA-N9 was investigated. We found that CGA-N9 induced the depolarization of the cell membrane and uptake of calcium ions into the cytosol and mitochondria. With the disruption of the mitochondrial membrane potential, the generation of intracellular reactive oxygen species (ROS) increased. Accordingly, we assessed apoptotic processes in Candida tropicalis cells post-treatment with CGA-N9 and found cytochrome c leakage, chromatin condensation and DNA degradation. The interaction of CGA-N9 with DNA in vitro showed that CGA-N9 did not degrade DNA but bound to DNA via an electrostatic interaction. In conclusion, CGA-N9 exhibits antifungal activity by inducing apoptosis in C. tropicalis.
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