期刊
AVIAN PATHOLOGY
卷 49, 期 2, 页码 144-152出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/03079457.2019.1687844
关键词
Avian pathogenic Escherichia coli; ybjx; miR-133b; KBTBD11; metabolic pathways; pathogenicity
资金
- Natural Science Foundation of China [31802161, 31772707, 31972644]
- National Key R&D Program of China [2018YFD0500101]
- Anhui Provincial Sci-Tech Major Project [17030701061]
ybjX gene mutation decreased the pathogenicity of the avian pathogenic Escherichia coli strain, AE17. However, the associated regulatory mechanism of ybjX remains unknown. In this study, we examined the bactericidal activity of chicken serum and blood, as well as bacterial survival in HD11 macrophages. We compared the transcriptome of ybjX mutations with those of the wild strain and studied the effects of ybjX on miRNA expression in the spleen. Our findings revealed that the mutant strain, Delta ybjX, had a lower resistance to chicken serum and blood, as well as lower bacterial survival in HD11 macrophages than AE17. RNA sequencing analyses showed that the ybjX mutation reduced stress resistance by down-regulating mRNAs in metabolic pathways. Infection with the ybjX mutant strain caused changes in the splenic miRNA profile. We verified Kelch repeat and BTB domain-containing protein 11 to be the target of miR-133b. Together, these findings suggest that the ybjX mutation reduces serum, blood, and environmental stress resistance by down-regulating the mRNA in metabolic pathways.
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