期刊
ARCHIVES OF ORAL BIOLOGY
卷 107, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2019.104514
关键词
Doxycycline; P. gingivalis-LPS; Adenosine triphosphate; NLRP3 inflammasome; IL-1 beta; Periodontitis
资金
- National Natural Science Foundation of China [81401526]
- Scientific Research Foundation of Qingdao University [DC1900009689]
- Natural Science Foundation of Shandong Province, China [ZR2019QC007, ZR2019MH003]
Objective: To investigate the effect of adenosine triphosphate (ATP) on inflammasome activation by Porphyromonas gingivalis-lipopolysaccharide (P. gingivalis-LPS) stimulation and the anti-inflammatory eff ;ect of doxycycline (Dox) in human gingival fibroblasts (HGFs). Design: The optimal concentration of P. gingivalis-LPS (1.0 mu g/mL) for cellular viability was determined by observing cell morphology and measuring the amount of formazan and the expression of pro-caspase-1. The expression of genes and proteins related to the NAcht Leucine-rich repeat Protein 3 (NLRP3) inflammasome, including NLRP3, apoptosis-associated speck-like protein containing CARD (ASC), caspase-1 and its activated forms, and the inflammatory factor interleukin-1 beta (IL-1 beta) and its activated forms were measured. Results: The NLRP3 inflammasome (i.e., NLRP3, ASC, caspase-1) was not affected by stimulation with P. gingivalis-LPS or ATP. However, a combination of P. gingivalis-LPS and ATP significantly enhanced inflammasome activation and IL-1 beta production at the gene and protein levels as measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively. Furthermore, doxycycline addition markedly inhibited inflammasome activation and IL-1 beta production induced by a combination of P. gingivalis-LPS and ATP. Conclusions: LPS, ATP, and doxycycline play critical roles in regulating host immune responses. This evidence provides guidance for the application of tetracycline drugs for the clinical treatment of periodontal disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据