期刊
AQUACULTURE
卷 520, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2019.734670
关键词
Marine fungi; Vibrio harveyi; Antibacterial substances; Isolation
资金
- National Key Research and Development Program of China [2018YFC0310900]
- National Natural Science Foundation of China [41776168, 41706167]
- Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources [NBHY-2017-P2]
- Zhejiang Provincial Public Welfare Technology Program [LGC19B020002]
- Natural Science Foundation of Ningbo [2018A610303]
- National 111 Project of China [D16013]
- Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Development Fund
- K.C. Wong Magna Fund in Ningbo University
- Ningbo Sci. & Tech. Projects for Common Wealth [2017C10016]
In this study, a fungal strain LS116 with antibacterial activity against aquatic pathogen Vibrio harveyi was screened out from sponge-associated fungi by Oxford cup method. According to its morphological characteristics and internal transcribed spacer (ITS) analysis, the active strain belonged to the genus Aspergillus. A bioactivity-guided approach was conducted to identify the antibacterial substances in the culture extract of strain LS116, a new C23 steroid with bicyclo [4.4.1]A/B ring, named aspergillsteroid A (1), and a known C25 steroid with the same structure skeleton, neocyclocitrinol B (2), were isolated and purified by column chromatography and HPLC (high-performance liquid chromatography) for the first time. Their chemical structures were determined by HRESIMS (high-resolution electrospray ionization mass spectroscopy) spectrometry, NMR (nuclear magnetic resonance) spectroscopy, and CD (circular dichroism). Among them, compound 1 displayed significant antibacterial activity against V. harveyi with a MIC value of 16 mu g/mL. The study demonstrated that compound 1 can be considered as a new promising agent for the control of aquatic disease in the future.
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