期刊
APPLIED RADIATION AND ISOTOPES
卷 153, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.apradiso.2019.108808
关键词
Non-Hodgkin's lymphoma; Tc- 99m-Hynic-F(ab ')(2)-Rituximab; Tc-99m-Hynic-F(ab ')-Rituximab; Radioimmunoscintigraphic agent
This study was to evaluate the potential of Tc-99m-Hynic-fragments of Rituximab as radioimmunoscintigraphic agents for diagnosis of patients with Non-Hodgkin's Lymphoma (NHL). Rituximab was digested with immobilized pepsin and papain to yield F(ab')(2) -Rituximab and F(ab')-Rituximab fragments respectively. Purified fragments were characterized by SE-HPLC and SDS-PAGE and subsequently radiolabeled with technetium-99m using Hynic as bifunctional chelator. The Tc-99m-Hynic-F(ab')(2)-Rituximab and Tc-99m-Hynic-F(ab')-Rituximab exhibited good in-vitro stability and specificity to Raji cells. Biodistribution studies demonstrated rapid pharmacokinetics and clearance predominantly through renal route.
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