期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 190, 期 4, 页码 1457-1469出版社
SPRINGER
DOI: 10.1007/s12010-019-03194-9
关键词
Chlamydomonas reinhardtii; Phytoene-beta-carotene synthase; Red yeast; beta-Carotene; Lutein
资金
- Department of Biotechnology, Ministry of Science & Technology, Government of India [BT/PR13796/PBD/26/139/2010]
- Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi
The metabolic engineering of Chlamydomonas reinhardtii, one of the fastest-growing microalgae, is a potential alternative for enhanced carotenoid productivity. CrtYB (phytoene-beta-carotene synthase - PBS) gene from red yeast Xanthophyllomyces dendrorhous encodes for a bifunctional enzyme that harbours both phytoene synthase (psy) and lycopene cyclization (lcyb) activities. Heterologous expression of this bifunctional PBS gene led to 38% enhancement in beta-carotene along with 60% increase in the lutein yields under low light conditions of 75 mu mol photons m(-2) s(-1). Short Duration-High Light induction strategy led to overall 72% and 83% increase in beta-carotene and lutein yield reaching up to 22.8 mg g(-1) and 8.9 mg g(-1), respectively. This is the first report of expression of heterologous bifunctional PBS gene resulting in simultaneous enhancement in beta-carotene and lutein content in phototrophic engineered cells.
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