期刊
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
卷 113, 期 1, 页码 13-20出版社
SPRINGER
DOI: 10.1007/s10482-019-01289-1
关键词
Lysobacter; Novel species; 16S rRNA gene; Biochemical analyses
类别
资金
- National Science and Technology Major Project [2018ZX10101004-003-003]
- Ministry of Science and Technology of China [2010ZX09401-403]
A Gram-stain negative, rod-shaped bacterial, catalase and oxidase positive strain (83-4(T)) that formed yellow colonies was isolated from human Meibomian gland secretions. Strain 83-4(T) belongs to the genus Lysobacter according to phylogenetic analysis based on 16S rRNA gene sequences. The DNA G+C content was 67.1 mol%. The circular genome was 2.6 Mb, which contained 2431 protein-coding sequences, 75 pseudogenes, 46 tRNAs, 3 rRNAs and 4 ncRNAs. A bacteriocin cluster and aryl polyene cluster were also found in the genome. The average nucleotide identity value was 79.6% between isolate 83-4(T) and the closely related type strain Lysobacter tolerans UM1(T). The estimated DNA-DNA hybridization value between strain 83-4(T) and L. tolerans UM1(T) was 41.6%. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. Iso-C-15:0, iso-C-11:0 3-OH, iso-C-11: 0 and summed feature 9 (iso-C-17:1 omega 9c) were the major fatty acids. Ubiquinone (Q-8) was the only respiratory quinone. Therefore, based on the data of phylogenetic analysis, chemotaxonomical and biochemical analyses, it is concluded that strain 83-4(T) represents a novel species of the genus Lysobacter with the name of Lysobacter oculi sp. nov. The type strain is 83-4(T) (= CGMCC 1.13464(T)= NRBC 113451(T)).
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