期刊
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 35
卷 35, 期 -, 页码 683-701出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev-cellbio-100818-125320
关键词
super-resolution imaging; gel embedding; tissue expansion; expansion microscopy
Expansion microscopy (ExM) is a physical form of magnification that increases the effective resolving power of any microscope. Here, we describe the fundamental principles of ExM, as well as how recently developed ExM variants build upon and apply those principles. We examine applications of ExM in cell and developmental biology for the study of nanoscale structures as well as ExM's potential for scalable mapping of nanoscale structures across large sample volumes. Finally, we explore how the unique anchoring and hydrogel embedding properties enable postexpansion molecular interrogation in a purified chemical environment. ExM promises to play an important role complementary to emerging live-cell imaging techniques, because of its relative ease of adoption and modification and its compatibility with tissue specimens up to at least 200 mu m thick.
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