期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 49, 页码 17827-17833出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201911025
关键词
near-infrared light; genome engineering; photon upconversion; singlet-to-triplet absorption; triplet-triplet annihilation
资金
- JSPS KAKENHI [JP25220805, JP17H04799, JP16H06513, JP16H00844]
- PRESTO program on Molecular Technology and Creation of New Functions from JST [JPMJPR14KE, JPMJPR14K1]
- Izumi Science and Technology Foundation
- Asahi Glass Foundation
- JSPS postdoctoral fellowships for foreign researchers
Photon upconversion (UC) from near-infrared (NIR) light to visible light has enabled optogenetic manipulations in deep tissues. However, materials for NIR optogenetics have been limited to inorganic UC nanoparticles. Herein, NIR-light-triggered optogenetics using biocompatible, organic TTA-UC hydrogels is reported. To achieve triplet sensitization even in highly viscous hydrogel matrices, a NIR-absorbing complex is covalently linked with energy-pooling acceptor chromophores, which significantly elongates the donor triplet lifetime. The donor and acceptor are solubilized in hydrogels formed from biocompatible Pluronic F127 micelles, and heat treatment endows the excited triplets in the hydrogel with remarkable oxygen tolerance. Combined with photoactivatable Cre recombinase technology, NIR-light stimulation successfully performs genome engineering resulting in the formation of dendritic-spine-like structures of hippocampal neurons.
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