期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 2, 页码 700-703出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201911664
关键词
dendrimers; DNA bricks; DNA nanotechnology; nanostructures; self-assembly
资金
- National Institutes of Health [1DP1GM133052]
- Office of Naval Research [N00014-18-1-2549]
- National Science Foundation [1540214]
- Wyss Institute's Immune Materials Platform
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1540214] Funding Source: National Science Foundation
The use of DNA-based nanomaterials in biomedical applications is continuing to grow, yet more emphasis is being put on the need for guaranteed structural stability of DNA nanostructures in physiological conditions. Various methods have been developed to stabilize DNA origami against low concentrations of divalent cations and the presence of nucleases. However, existing strategies typically require the complete encapsulation of nanostructures, which makes accessing the encased DNA strands difficult, or chemical modification, such as covalent crosslinking of DNA strands. We present a stabilization method involving the synthesis of DNA brick nanostructures with dendritic oligonucleotides attached to the outer surface. We find that nanostructures assembled from DNA brick motifs remain stable against denaturation without any chemical modifications. Furthermore, densely coating the outer surface of DNA brick nanostructures with dendritic oligonucleotides prevents nuclease digestion.
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