期刊
ANALYTICAL CHEMISTRY
卷 91, 期 24, 页码 16010-16016出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b04783
关键词
-
资金
- National Natural Science Foundation [21575090]
- High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [CITTCD20190330]
- Scientific Research Project of Beijing Educational Committee [KM201810028008]
- Youth Innovative Research Team of Capital Normal University
- Capacity Building for Sci-Tech Innovation -Fundamental Scientific Research Funds [19530050179, 025185305000/195]
Continuous recording of the dynamic changes of multiple physiologically neurochemicals in vivo is vital to understand the molecular basis of brain functions. For instance, disentangling the complicated interrelationship between ascorbic acid (AA) and copper ions (Cu2+) in signal transduction and homeostasis remains essential in understanding the pathology of hypoxia damage and neurodegenerative diseases. Unfortunately, to the best of our knowledge, there is still no report regarding the development of online and in vivo electrochemical or optical methods that can simultaneously measure AA and Cu2+ due to the limitation of different detection mechanisms and environmental conditions. In this work, we report a parallel dual-channel online optical detection platform (OODP) for the continuous and simultaneous monitoring of AA and Cu2+ in a living rat brain by integrating a capillary-based microfluidic system as well as an optical detector built under a bright-field microscope. Two kinds of colorimetric sensors, oxidized tetramethylbenzidine (oxTMB) and dopamine functionalized silver nanoparticles (DA-AgNPs), were used to recognize AA and Cu2+ in physically isolated detection channels. Combined with in vivo microdialysis sampling, the dual-channel OODP exhibited a wide linearity for AA and Cu2+ detection in the ranges of 0.5 mu M to 100 mu M and 0.1 mu M to 10 mu M, respectively. Compared to online electrochemical systems, this dual-channel OODP shows the following advantages: (1) isolated detection positions for two species, which can easily avoid crosstalk, and (2) a small dead-volume of 0.0113 mu L in the detector, which obviously improves the time resolution. Along with its high stability, selectivity, and a near real-time response, the constructed dual-channel OODP can be successfully used to monitor cerebral AA and Cu2+ alterations simultaneously in a living rat brain.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据