期刊
ANALYTICAL CHEMISTRY
卷 91, 期 22, 页码 14538-14544出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b03556
关键词
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资金
- National Science Foundation of China [21775159, 21305153, 21435008, 91743201]
- National Basic Research Program of China [2015CB932003]
- Sanming Project of Medicine in Shenzhen [SZsm201811070]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB14030200]
Silver is a common heavy metal, and the detection of silver ion (Ag+) is of great importance because of its wide application and hazardous effect on the environment and human health. However, it is a great challenge to produce a large fluorescence anisotropy (FA) change for small molecules (e.g, Ag+ Herein, we describe a novel fluorescence anisotropy reduction approach for the sensitive and specific detection of Ag+. The feasibility of this method is demonstrated through screening a number of guanine-rich oligonucleotide probes. By selectively labeling the oligonucleotides with a single fluorophore tetramethylrhodamine (TMR), the reduction in FA response is associated with the conformation change from the unfolded to a hairpin-like folded structure by inducing formation of the intermolecular G-Ag+-G base pair, which diminishes the interaction between guanine and TMR by photoinduced electron transfer (PET). The change in FA allows the selective detection of Ag+ at a concentration as low as 0.5 nM with a dynamic range from 2.0 to 100 nM. The interference from the other 14 metal ions with a 100-fold even to a 1000-fold excess amount is negligible. This simple and cost-effective probe was further explored to determine cysteine (Cys) based on competing with a guanine-rich oligonucelotide for Ag+-binding.
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