期刊
ANALYTICAL CHEMISTRY
卷 91, 期 21, 页码 14066-14073出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b03796
关键词
-
资金
- National Key Scientific Instrument and Equipment Development Project of China [21627809]
- National Natural Science Foundation of China [21575050, 21505051, 21777056]
- Special Foundation for Taishan Scholar Professorship of Shandong Province [ts201712052]
- Jinan Scientific Research Leader Workshop Project [2018GXRCO24]
Electrocheluminescence (ECL) immunoassay amplified by coreaction accelerators has experienced major breakthroughs in ultrasensitive detection of biomarkers. Herein, CeO2/SnS2 heterostructures were synthesized and applied as a novel coreaction accelerator to enhance the ECL efficiency of the luminol dissolved O-2 system for the first time. Benefiting from the well-matched lattice spacing, ultrafine CeO2 nanoparticles (NPs) were grown in situ on layered SnS2 nanosheets (NSs) with improved dispersion. CeO2/SnS2 as an electroactive substrate can remarkably accelerate the generation of abundant superoxide anion radicals (O-2(center dot-)) to react with luminol anion radical (L center dot-), achieving about 2-fold stronger ECL intensity than that of pure CeO2 NPs. To avoid harsh chemical synthesis of conventional ECL labels and simplify the antibody conjugation process, ferritin (Ft) was served as a natural nanocarrier to immobilize luminol molecules (Lum@Ft) via a one-step linkage, whose protein nanocage can easily connect with the detection antibody. Moreover, a robust site-oriented immobilization strategy using HWRGWVC heptapeptide as specific capturer was further adopted to maintain the bioactivity of the capture antibody on the amine-functionalized CeO2/SnS2 surface, which promoted the incubation efficiency markedly. On account of this advanced sensing strategy, a brand new biosensor was constructed for the accurate detection of heart failure biomarkers, which performed with favorable linearity in the range of 0.0001-50 ng/mL and achieved the detection limit of 36 fg/mL.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据