期刊
ANALYTICA CHIMICA ACTA
卷 1098, 期 -, 页码 117-124出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.11.027
关键词
Photo-heat conversion immunoassay; Carbohydrate antigen 19-9; Prussian blue nanoparticles; CaCO3 microspheres; 3D-printed device
资金
- Fund for Excellent Youth in Peking Union Medical College Hospital (PUMCH-2018)
A near-infrared (NIR) photothermal immunoassay was designed for the sensitive monitoring of CA 19-9 on the capture antibody-coated microplate, coupling a 3D-printed device with a digital thermometer in this work. Prussian blue nanoparticles (PBNPs)-encapsulated CaCO3 microspheres were not only utilized for labeling of detection antibody, but used as the photo-heat conversion materials for the signal amplification. With the sandwiched immunocomplex, the as-released PBNPs under acidic conditions adsorbed and converted NIR-light wavelength to heat under 808-nm laser irradiation, thereby resulting in the temperature change of the detection solution. Under optimum conditions, a linear range from 1.0 U mL(-1) to 100 U mL(-1) and a detection limit of 0.83 U mL(-1) were acquired for the CA 19-9 detection on the portable photothermal immunosensing platform with PBNP-CaCO3 -labeling system. Relative standard deviations for reproducibility were <= 9.7% for intra-assay and <= 11.9% for inter-assay. High specificity, long-time storage stability (>10 months) and good accuracy (relative to gold standard with commercial human ELISA kit) with the photothermal immunoassay were encountered for the evaluation of target CA 19-9 in complex system. (C) 2019 Elsevier B.V. All rights reserved.
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