期刊
ANALYTICA CHIMICA ACTA
卷 1079, 期 -, 页码 129-138出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.05.073
关键词
Acoustofluidics; Acoustic streaming; ELISA; Immunoassay
资金
- West Virginia University Start-up fund
- Don and Linda Brodie Resource Fund for Innovation
Bead-based ELISA in microfluidics is a promising platform for reducing the reagent consumption and improving assay throughput due to its fast binding kinetics and low reagent consumption. Current microfluidic bead-based immunoassay mainly relies on magnetic and centrifugal forces to manipulate microparticles to complete an assay protocol. Here we report an acoustic streaming-based microfluidic method that can perform all the fluid and particle operations of bead-based ELISA. A series of sharp-edge structures are used to generate a long-range coupled acoustic streaming that enables simultaneous particle trapping and active molecular exchange in a dead-end microchannel. The device achieved > 99% of molecular exchange in 4 min, while maintaining a particle trapping efficiency of 85%. This acoustofluidic-based method demonstrates all three key operations in a bead-based immunoassay: (1) Beads immobilization; (2) Active mixing of fluid for bead/target binding; (3) Active molecular exchange for reagent loading and washing. Finally, on-chip quantitative detection of biomarker IL-6 with a limit of detection similar to 50 pg/mL is achieved using this platform including an enzymatic signal amplification step. The small footprint, simple setup, and continuous flow operation of the acoustic streaming-based method makes it an attractive platform for continuous flow bead-based immunoassay. (C) 2019 Elsevier B.V. All rights reserved.
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