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注意：仅列出部分参考文献，下载原文获取全部文献信息。Inteins as tools for tagless and traceless protein purification
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Conditional Protein Splicing Switch in Hyperthermophiles through an Intein-Extein Partnership
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Light Activation of Protein Splicing with a Photocaged Fast Intein
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Protein Splicing: How Inteins Escape from Precursor Proteins
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Intein Applications: From Protein Purification and Labeling to Metabolic Control Methods
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Fusion protein linkers: Property, design and functionality
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ADVANCED DRUG DELIVERY REVIEWS (2013)
A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification
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Recent progress in intein research: from mechanism to directed evolution and applications
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Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification
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Spontaneous Proton Transfer to a Conserved Intein Residue Determines On-Pathway Protein Splicing
Brian Pereira et al.
JOURNAL OF MOLECULAR BIOLOGY (2011)
Active protein aggregates induced by terminally attached self-assembling peptide ELK16 in Escherichia coli
Wei Wu et al.
MICROBIAL CELL FACTORIES (2011)
Streamlined protein expression and purification using cleavable self-aggregating tags
Lei Xing et al.
MICROBIAL CELL FACTORIES (2011)
Backbone Dynamics and Global Effects of an Activating Mutation in Minimized Mtu RecA Inteins
Zhenming Du et al.
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The potential role of self-cleaving purification tags in commercial-scale processes
Baley A. Fong et al.
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Selection and Structure of Hyperactive Inteins: Peripheral Changes Relayed to the Catalytic Center
Kaori Hiraga et al.
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Highly Conserved Histidine Plays a Dual Catalytic Role in Protein Splicing: A pKa Shift Mechanism
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Modulation of intein activity by its neighboring extein substrates
Gil Amitai et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009)
Addressing the numbers problem in directed evolution
Manfred T. Reetz et al.
CHEMBIOCHEM (2008)
Control of protein phosphorylation with a genetically encoded photocaged amino acid
Edward A. Lemke et al.
NATURE CHEMICAL BIOLOGY (2007)
Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue
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JOURNAL OF MOLECULAR BIOLOGY (2007)
Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes
Manfred T. Reetz et al.
NATURE PROTOCOLS (2007)
A genetically encoded photocaged tyrosine
A Deiters et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2006)
Minimization and stabilization of the Mycobacterium tuberculosis recA intein
K Hiraga et al.
JOURNAL OF MOLECULAR BIOLOGY (2005)
Very fast empirical prediction and rationalization of protein pKa values
H Li et al.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2005)
Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test
MT Reetz et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2005)
A conformational flexibility scale for amino acids in peptides
F Huang et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2003)
Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor
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BIOTECHNOLOGY PROGRESS (2000)
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