期刊
AGING-US
卷 11, 期 20, 页码 9043-9059出版社
IMPACT JOURNALS LLC
DOI: 10.18632/aging.102372
关键词
bioinformatics; brain science; drug treatment; functional pituitary adenomas; prognosis
资金
- National Natural Science Foundation of China [81672505, 81772684]
- S&T Development Planning Program of Jilin Province [20160101086JC, 20160312017ZG, 20180101152JC]
- Jilin Provincial Education Department 13th Five-Year Science and Technology Project [JJKH20180191KJ]
- Interdisciplinary Innovation Project of The First Hospital of Jilin University [JDYYJC001]
We tested whether the drugs T5224, RSPO2, and AZD5363 exert therapeutic effects against functioning pituitary adenoma (FPA). We analysed the gene expression profiles of four FPA mRNA microarray datasets (GSE2175, GSE26966, GSE36314, and GSE37153) from the Gene Expression Omnibus database and identified genes differentially expressed in FPA vs control tissues. We then carried out Gene Ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction network analyses. We also measured the difference in expression of hub genes between human normal pituitary cells and FPA cells using qRT-PCR. Our in vitro colonyformation and MTT assays showed that cell viability, number, and the size of clonogenicities were all lower in the presence of T5224, RSPO2, or AZD536 than in controls. Moreover, flow cytometry experiments showed that the incidence of apoptosis was higher in the presence of T5224, RSPO2, or AZD5363 than among controls, and was increased by increasing the doses of the drugs. This suggests these drugs could be used as therapeutic agents to treat FPA. Finally, we found that cFos, WNT5A, NCAM1, JUP, AKT3, and ADCY1 are abnormally expressed in FPA cells compared to controls, which highlights these genes as potential prognostic and/or therapeutic targets.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据