期刊
AGING-US
卷 11, 期 20, 页码 8860-8878出版社
IMPACT JOURNALS LLC
DOI: 10.18632/aging.102307
关键词
long non-coding SBF2-AS1; competing endogenous RNA; microRNA-142-3p; gemcitabine resistance; pancreatic cancer
资金
- National Nature Science Foundation of China [81973702, 81673749, 81973629, 81973616, 81930115, 81673746, 81603439, 81873122]
- Development Action Plan of T.C.M. of Shanghai Municipal Commission Of Health and Family Planning [ZY3-CCCX-3-3031, ZY3-LCPT-2-1001]
- Medical Guide Project of the Shanghai Municipal Commission for Science and Technology [15401932400]
- Xinglin star plan of Shanghai Municipal Commission of Health and Family Planning [ZY3-RCPY-2-2023]
- Project of Shanghai Municipal Health Commission [2014JP010B]
Objective: This study is implemented to probe into the function of lncRNA SBF2-AS1 as a competing endogenous RNA (ceRNA) to sponge microRNA-142-3p (miR-142-3p) in modulating TWF1 expression in the gemcitabine resistance of pancreatic cancer. Results: LncRNA SBF2-AS1 was highly expressed in pancreatic cancer tissues and cells. SBF2-AS1 was found to be associated with gemcitabine resistance in pancreatic cancer. Knock-down of SBF2-AS1 inhibited proliferation, epithelial-mesenchymal transition, while promoting apoptosis of gemcitabine resistant pancreatic cancer cells. SBF2-AS1 inhibited the expression of TWF1 by competitively binding with miR-142-3p in pancreatic cancer. Conclusion: Our study demonstrates that knock-down of SBF2-AS1 inhibits the expression of TWF1 by competitively binding with miR-142-3p to induce gemcitabine resistance in pancreatic cancer. Methods: Expression of SBF2-AS1 was tested in pancreatic cancer tissues and cells. Construction of AsPC-1/GEM and PANC-1/GEM cells with low expression of SBF2-AS1 was performed to determine the biological behaviors of drug-resistant cells. AsPC-1 and PANC-1 cells expressing SBF2-AS1 and/or miR-142-3p were constructed and treated with different concentrations of gemcitabine to detect the sensitivity of the cells to gemcitabine. The binding relationship between SBF2-AS1 and miR-142-3p and between miR-142-3p and TWF1 were determined.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据