期刊
ADVANCED FUNCTIONAL MATERIALS
卷 30, 期 1, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201908349
关键词
bioprinting; endothelialization; hydrogels; microfluidics; printability
类别
资金
- EPSRC Programme Grant Engineering growth factor microenvironments-a new therapeutic paradigm for regenerative medicine [EP/P001114/1]
- Medical Research Council (MRC) [MR/S00551X/1]
- UK Regenerative Medicine Platform Acellular/Smart Materials-3D Architecture [MR/R015651/1]
- Wellcome Trust Senior Investigator Award [098411/Z/12/Z]
- EPSRC [EP/P001114/1] Funding Source: UKRI
- MRC [MR/R015651/1, MR/S00551X/1] Funding Source: UKRI
Two major challenges of 3D bioprinting are the retention of structural fidelity and efficient endothelialization for tissue vascularization. Both of these issues are addressed by introducing a versatile 3D bioprinting strategy, in which a templating bioink is deposited layer-by-layer alongside a matrix bioink to establish void-free multimaterial structures. After crosslinking the matrix phase, the templating phase is sacrificed to create a well-defined 3D network of interconnected tubular channels. This void-free 3D printing (VF-3DP) approach circumvents the traditional concerns of structural collapse, deformation, and oxygen inhibition, moreover, it can be readily used to print materials that are widely considered unprintable. By preloading endothelial cells into the templating bioink, the inner surface of the channels can be efficiently cellularized with a confluent endothelial layer. This in situ endothelialization method can be used to produce endothelium with a far greater cell seeding uniformity than can be achieved using the conventional postseeding approach. This VF-3DP approach can also be extended beyond tissue fabrication and toward customized hydrogel-based microfluidics and self-supported perfusable hydrogel constructs.
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