Optimization of Antibacterial Efficacy of Noble-Metal-Based Core-Shell Nanostructures and Effect of Natural Organic Matter

Noble -metal-based nanomaterials made of less toxic metals have been utilized as potential antibacterial agents due to their distinctive oxidase-like activity. In this study, we fabricated core-shell structured PdpIr bimetallic nanomaterials with an ultrathin shell. Pd@Ir nanostructures show morphology-dependent bactericidal activity, in which Pd@Ir octahedra possessing higher oxidase-like activity exert bactericidal activity stronger than that of Pd@Ir cubes. Furthermore, our results reveal that the presence of natural organic matter influences the antibacterial behaviors of nanomaterials. Upon interaction with humic acid (HA), the Pd@Ir nanostructures induce an elevated level of reactive oxygen species, resulting in significantly enhanced bactericidal activity of the nanostructures. Mechanism analysis shows that the presence of HA efficiently enhances the oxidase-like activity of nanomaterials and promotes the cellular internalization of nanomaterials. We believe that the present study will not only demonstrate an effective strategy for improving the bactericidal activity of noble-metal-based nanomaterials but also provide an understanding of the antibacterial behavior of nanomaterials in the natural environment.