4.3 Article

Biosynthesis of phycocyanobilin in recombinant Escherichia coli

期刊

JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷 38, 期 2, 页码 529-538

出版社

SCIENCE PRESS
DOI: 10.1007/s00343-019-9060-6

关键词

combinational biosynthesis; phycocyanobilin; response surface methodology; antioxidant

资金

  1. National Natural Science Foundation of China [41906109]
  2. Key Research and Development Program of Yantai [Y933021011]

向作者/读者索取更多资源

The recombinant expression of phycocyanobilin (PCB) was carried out in Escherichia coli, and the best fermentation conditions of recombinant E. coli biosynthesized PCB are optimized in the response surface methodology to improve PCB production. The recombinant PCB is extracted, isolated, and purified by methanol and chloroform extraction. Recombinant PCB is validated in UV-vis spectroscopy, high-pressure liquid chromatography, and mass spectrometry. In addition, the anti-oxidant activities of the recombinant PCB are determined. The best induction conditions that optimized by Design Expert 8.0 software include: lactose concentration 4 mmol/L, induction temperature 24.69 degrees C, induction time 4.6 h, and induction duration 13.57 h, under which the PCB expression level reached approximately 13 mg PCB/L, which is more than four times of previously reported 3 mg PCB/L. The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm. The recombinant PCB has a good ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据