期刊
ACS OMEGA
卷 4, 期 9, 页码 13687-13695出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.9b01073
关键词
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资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [26249116, 16K18279, T15K142040, 19K15338]
- Japanese Government (Monbukagakusho: MEXT) scholarship
- Grants-in-Aid for Scientific Research [19K15338] Funding Source: KAKEN
A gold nanoparticle (AuNP) has a localized surface plasmon resonance peak depending on its size, which is often utilized for surface-enhanced Raman scattering (SERS). To obtain information on the cholesterol (Chol)-incorporated lipid membranes by SERS, AuNPs (5, 100 nm) were first functionalized by 1-octanethiol and then modified by lipids (AuNP@lipid). In membrane surface-enhanced Raman spectroscopy (MSERS), both signals from 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and Chol molecules were enhanced, depending on preparation conditions (size of AuNPs and lipid/AuNP ratio). The enhancement factors (EFs) were calculated to estimate the efficiency of AuNPs on Raman enhancement. The size of AuNP100nm@lipid was 152.0 +/- 12.8 nm, which showed an surface enhancement Raman spectrum with an EF2850 value of 111 +/- 9. The size of AuNP5nm@lipid prepared with a lipid/AuNP ratio of 1.38 x 10(4) (lipid molecule/particle) was 275.3 +/- 20.2 nm, which showed the highest enhancement with an EF2850 value of 131 +/- 21. On the basis of fluorescent probe analyses, the membrane fluidity and polarity of AuNP@lipid were almost similar to DOPC/Chol liposome, indicating an intact membrane of DOPC/Chol after modification with AuNPs. Finally, the membrane properties of AuNP@lipid systems were also discussed on the basis of the obtained MSERS signals.
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