期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
卷 1859, 期 5, 页码 731-743出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagrm.2016.03.008
关键词
Rpb4; Assembly; Stress; Stability; mRNA
资金
- Spanish Ministry of Economy and Competitiveness
- MINECO
- FEDER [BFU2013-48643-C3-2-P, BFU2013-48643-C3-3-P]
- Junta de Andalucia [BI0258, PI10-CVI6521]
- Generalitat Valenciana (GVPrometeo II)
- Ministerio de Education Y Ciencia (MEC)
- University of Jaen
- Santiago Grisolia fellowship from the GV
The RPB1 mutants in the foot region of RNA polymerase II affect the assembly of the complex by altering the correct association of both the RpbG and the Rpb4/7 dimer. Assembly defects alter both transcriptional activity as well as the amount of enzyme associated with genes. Here, we show that the global transcriptional analysis of foot mutants reveals the activation of an environmental stress response (ESR), which occurs at a permissive temperature under optimal growth conditions. Our data indicate that the ESR that occurs in foot mutants depends mostly on a global post-transcriptional regulation mechanism which, in turn, depends on Rpb4-mRNA imprinting. Under optimal growth conditions, we propose that Rpb4 serves as a key to globally modulate mRNA stability as well as to coordinate transcription and decay. Overall, our results imply that post-transcriptional regulation plays a major role in controlling the ESR at both the transcription and mRNA decay levels. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据