期刊
HEREDITAS
卷 156, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s41065-019-0105-9
关键词
Chromatin accessibility; Tn5 transposase; ATAC-seq; Promoter; Enhancer
资金
- China Postdoctoral Science Foundation [2017 M622053]
- National Natural Science Foundation of China [81471152, 31771141]
Background Chromatin accessibility is crucial for gene expression regulation in specific cells and in multiple biological processes. Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) is an effective way to reveal chromatin accessibility at a genome-wide level. Through ATAC-seq, produced reads from a small number of cells reflect accessible regions that correspond to nucleosome positioning and transcription factor binding sites, due to probing hyperactive Tn5 transposase to DNA sequence. Conclusion In this review, we summarize both principle and features of ATAC-seq, highlight its applications in basic and clinical research. ATAC-seq has generated comprehensive chromatin accessible maps, and is becoming a powerful tool to understand dynamic gene expression regulation in stem cells, early embryos and tumors.
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