期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1858, 期 12, 页码 3105-3112出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2016.09.019
关键词
Membrane; Transporters; beta-barrel proteins; TonB; Nanodiscs; Scintillation proximity assay
资金
- Natural Sciences and Engineering Research Council of Canada [DG-40042]
TonB-dependent transporters are (beta-barrel outer membrane proteins occluded by a plug domain. Upon ligand binding, these transporters extend a periplasmic motif termed the TonB box. The TonB box permits the recruitment of the inner membrane protein complex TonB-ExbB-ExbD, which drives import of ligands in the cell periplasm. It is unknown precisely how the plug domain is moved aside during transport nor have the intermediate states between TonB recruitment and plug domain movement been characterized biochemically. Here we employ nanodiscs, native gel electrophoresis, and scintillation proximity assays to determine the binding kinetics of vitamin B-12 to BtuB. The results show that ligand-bound BtuB recruits a monomer of TonB (TonB(Delta 1-31)), which in turn increases retention of vitamin B-12 within the transporter. The TonB box and the extracellular residue valine 90 that forms part of the vitamin B-12 binding site are essential for this event. These results identify a novel step in the TonB-dependent transport process. They show that TonB binding to BtuB trap the ligand, possibly until the ExbB-ExbD complex is activated or recruited to ensure subsequent transport. (C) 2016 Elsevier B.V. All rights reserved.
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