期刊
WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY
卷 11, 期 9, 页码 705-716出版社
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.4251/wjgo.v11.i9.705
关键词
MicroRNA-331; Musashi1; Tumor growth; Metastasis; Gastric cancer
BACKGROUND The molecular mechanisms involved in microRNAs (miRNAs) have been extensively investigated in gastric cancer (GC). However, how miR-331 regulates GC pathogenesis remains unknown. AIM To illuminate the effect of miR-331 on cell metastasis and tumor growth in GC. METHODS The qRT-PCR, CCK8, Transwell, cell adhesion, Western blot, luciferase reporter and xenograft tumor formation assays were applied to explore the regulatory mechanism of miR-331 in GC. RESULTS Downregulation of miR-331 associated with poor prognosis was detected in GC. Functionally, miR-331 suppressed cell proliferation, metastasis and tumor growth in GC. Further, miR-331 was verified to directly target musashi1 (MSI1). In addition, miR-331 inversely regulated MSI1 expression in GC tissues. Furthermore, upregulation of MSI1 weakened the inhibitory effect of miR-331 in GC. CONCLUSION miR-331 inhibited development of GC through targeting MSI1, which may be used as an indicator for the prediction and prognosis of GC.
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