期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1857, 期 10, 页码 1694-1704出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2016.07.006
关键词
Anaerobic ammonium oxidation (anammox); Complexome profiling; Blue Native gel electrophoresis; Respiratory complexes
资金
- Netherlands Organization for Scientific Research (NWO) [ALW2PJ/08021, 863.11.003]
- European Research Council [ERC 640422, ERC 232937, ERC 339880]
Electron transport, or oxidative phosphorylation, is one of the hallmarks of life. To this end, prokaryotes evolved a vast variety of protein complexes, only a small part of which have been discovered and studied. These protein complexes allow them to occupy virtually every ecological niche on Earth. Here, we applied the method of proteomics-based complexome profiling to get a better understanding of the electron transport systems of the anaerobic ammonium-oxidizing (anammox) bacteria, the N-2-producing key players of the global nitrogen cycle. By this method nearly all respiratory complexes that were previously predicted from genome analysis to be involved in energy and cell carbon fixation were validated. More importantly, new and unexpected ones were discovered. We believe that complexome profiling in concert with (meta)genomics offers great opportunities to expand our knowledge on bacterial respiratory processes at a rapid and massive pace, in particular in new and thus far poorly investigated non-model and environmentally-relevant species. (C) 2016 Elsevier B.V. All rights reserved.
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