期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1857, 期 4, 页码 350-358出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2016.01.006
关键词
Mitochondria; FOF1 ATP synthase; Intramitochondrial cAMP; sAC
资金
- MIUR (Progetto FIRB futuro in ricerca) [RBFR0813Z5]
- MIUR (FIRB-MERIT 'Molecular basis in ageing-related degenerative syndrome') [RBNE08HWLZ]
The present study shows that in isolated mitochondria and myoblast cultures depletion of cAMP, induced by sAC inhibition, depresses both ATP synthesis and hydrolysis by the FOF1 ATP synthase (complex V) of the oxidative phosphorylation system (OXPHOS). These effects are accompanied by the decrease of the respiratory membrane potential, decreased level of FOF1 connecting subunits and depressed oligomerization of the complex. All these effects of sAC inhibition are prevented by the addition of the membrane-permeant 8-Br-cAMP. These results show, for the first time, that cAMP promotes ATP production by complex V and prevents, at the same time, its detour to a mitochondria] membrane leak conductance, which is involved in cell death. (C) 2016 Elsevier B.V. All rights reserved.
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