4.6 Article

Encephalopathy induced by Alzheimer brain inoculation in a non-human primate

期刊

出版社

BMC
DOI: 10.1186/s40478-019-0771-x

关键词

Alzheimer's disease; beta-Amyloid pathology; Cerebral atrophy; Cognitive impairment; Microcebus murinus; Mouse; Neurodegenerative disease; Neuronal function; Prion; Tau pathology

资金

  1. France-Alzheimer Association
  2. Fondation Plan Alzheimer
  3. CEA Bottom-Up program

向作者/读者索取更多资源

Alzheimer's disease is characterized by cognitive alterations, cerebral atrophy and neuropathological lesions including neuronal loss, accumulation of misfolded and aggregated beta-amyloid peptides (A beta) and tau proteins. Iatrogenic induction of A beta is suspected in patients exposed to pituitary-derived hormones, dural grafts, or surgical instruments, presumably contaminated with A beta. Induction of A beta and tau lesions has been demonstrated in transgenic mice after contamination with Alzheimer's disease brain homogenates, with very limited functional consequences. Unlike rodents, primates naturally express A beta or tau under normal conditions and attempts to transmit Alzheimer pathology to primates have been made for decades. However, none of earlier studies performed any detailed functional assessments. For the first time we demonstrate long term memory and learning impairments in a non-human primate (Microcebus murinus) following intracerebral injections with Alzheimer human brain extracts. Animals inoculated with Alzheimer brain homogenates displayed progressive cognitive impairments (clinical tests assessing cognitive and motor functions), modifications of neuronal activity (detected by electroencephalography), widespread and progressive cerebral atrophy (in vivo MRI assessing cerebral volume loss using automated voxel-based analysis), neuronal loss in the hippocampus and entorhinal cortex (post mortem stereology). They displayed parenchymal and vascular A beta depositions and tau lesions for some of them, in regions close to the inoculation sites. Although these lesions were sparse, they were never detected in control animals. Tau-positive animals had the lowest performances in a memory task and displayed the greatest neuronal loss. Our study is timely and important as it is the first one to highlight neuronal and clinical dysfunction following inoculation of Alzheimer's disease brain homogenates in a primate. Clinical signs in a chronic disease such as Alzheimer take a long time to be detectable. Documentation of clinical deterioration and/or dysfunction following intracerebral inoculations with Alzheimer human brain extracts could lead to important new insights about Alzheimer initiation processes.

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