期刊
SCIENCE ADVANCES
卷 5, 期 9, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aaw2459
关键词
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资金
- Office of Naval Research Vannevar Bush Faculty Fellowship program [N000141612823]
- NSF CELL-MET [EEC-1647837]
- National Human Genome Research Institute of the NIH [RM1HG008525]
- GETTYLAB
- 3D Organ Engineering Initiative at the Wyss Institute
- U.S. Department of Defense (DOD) [N000141612823] Funding Source: U.S. Department of Defense (DOD)
Engineering organ-specific tissues for therapeutic applications is a grand challenge, requiring the fabrication and maintenance of densely cellular constructs composed of similar to 10(8) cells/ml. Organ building blocks (OBBs) composed of patient-specific-induced pluripotent stem cell-derived organoids offer a pathway to achieving tissues with the requisite cellular density, microarchitecture, and function. However, to date, scant attention has been devoted to their assembly into 3D tissue constructs. Here, we report a biomanufacturing method for assembling hundreds of thousands of these OBBs into living matrices with high cellular density into which perfusable vascular channels are introduced via embedded three-dimensional bioprinting. The OBB matrices exhibit the desired self-healing, viscoplastic behavior required for sacrificial writing into functional tissue (SWIFT). As an exemplar, we created a perfusable cardiac tissue that fuses and beats synchronously over a 7-day period. Our SWIFT biomanufacturing method enables the rapid assembly of perfusable patient- and organ-specific tissues at therapeutic scales.
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