期刊
CHEMISTRYSELECT
卷 4, 期 35, 页码 10270-10275出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/slct.201902327
关键词
Conformational changes; Ethyl-substituted Thioflavin T; Fluorescent probe; Human serum transferrin
Human serum transferrin (hTf) is found that can be used as novel drug active targeted vector, which have broad application prospects in the field of tumor-targeted therapeutics. A new method to recognize human transferrin (hTf) conformations was developed using ThT-E. ThT-E with three ethyl functional groups has better hydrophobicity than ThT, which can better recognize transferrin and achieve detection of the open conformation of Tf (apo-Tf) against the closed one (holo-Tf). In this study, ThT-E and ThT with similar main core structures were selected to evaluate the effect of heterocycles on hTf binding by spectroscopic methods, and a more excellent fluorescent probe ThT-E was selected for the recognition of transferrin conformation. And ThT-E has a detection limit of 0.27 mu M for apo-Tf. This study provides a theoretical basis for ThT-E as a biomolecular probe for detecting the mutual conversion of two conformational proteins.
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