An NBD-Based Mitochondrial Targeting Ratiometric Fluorescent Probe for Hydrogen Sulfide Detection

Hydrogen sulfide (H2S) is an important endogenous gaseous signaling molecule with multiple biological functions. In this study, an NBD-based ratiometric fluorescent probe (MR-1) was successfully designed and synthesized for selectively detection H2S. The probe exhibits high selectivity and fast emission intensity ratio changes in response to H2S in solution. The modification of triphenylphosphine in the probe enables it to be used for the imaging mitochondrial H2S in living HeLa cells.